Genetic analysis of two phenotypes in variovorax paradoxus strain EPS

The formation of biofilms is a complex process that involves activation of a wide array of genetic material within the cells found living within the biofilm. It has been shown that altered colony morphology has been associated with different biofilm levels within P. aureginosa. This analysis will provide further evidence of the strength of the colony morphology screen to isolate mutants with altered biofilm or swarming abilities

On the Confidence in Bit-Alias Measurement of Physical Unclonable Functions

Physical Unclonable Functions (PUFs) are modern solutions for cheap and secure key storage. The security level strongly depends on a PUF's unpredictability, which is impaired if certain bits of the PUF response tend towards the same value on all devices. The expectation for the probability of 1 at some position in the response, the Bit-Alias, is a state-of-the-art metric in this regard. However, the confidence interval of the Bit-Alias is never considered, which can lead to an overestimation of a PUF's unpredictability. Moreover, no tool is available to verify if the Bit-Alias is within given limits. This work adapts a method for the calculation of confidence intervals to Bit-Alias. It further proposes a statistical hypothesis test to verify if a PUF design meets given specifications on Bit-Alias or bit-wise entropy. Application to several published PUF designs demonstrates the methods' capabilities. The results prove the need for a high number of samples when the unpredictability of PUFs is tested. The proposed methods are publicly available and should improve the design and evaluation of PUFs in the future.Comment: Original publication at 2019 17th IEEE International New Circuits and Systems Conference (NEWCAS

Development of a Single Mechanical Seal Equipped With API Piping Plan 11/66a for Large Mainline Crude Oil Pipeline Pumps

Lectur

RRAM Based Random Bit Generation for Hardware Security Applications

© 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.Resistive random access memories (RRAMs) have arisen as a competitive candidate for non-volatile memories due to their scalability, simple structure, fast switching speed and compatibility with conventional back-end processes. The stochastic switching mechanism and intrinsic variability of RRAMs still poses challenges that must be overcome prior to their massive memory commercialization. However, these very same features open a wide range of potential applications for these devices in hardware security. In this context, this work proposes the generation of a random bit by means of simultaneous write operation of two parallel cells so that only one of them unpredictably switches its state. Electrical simulations confirm the strong stochastic behavior and stability of the proposed primitive. Exploiting this fact, a Physical Unclonable Function (PUF) like primitive is implemented based on modified 1 transistor - 1 resistor (1T1R) array structure.Peer ReviewedPostprint (published version

Coordinated surface activities in Variovorax paradoxus EPS

<p>Abstract</p> <p>Background</p> <p><it>Variovorax paradoxus </it>is an aerobic soil bacterium frequently associated with important biodegradative processes in nature. Our group has cultivated a mucoid strain of <it>Variovorax paradoxus </it>for study as a model of bacterial development and response to environmental conditions. Colonies of this organism vary widely in appearance depending on agar plate type.</p> <p>Results</p> <p>Surface motility was observed on minimal defined agar plates with 0.5% agarose, similar in nature to swarming motility identified in <it>Pseudomonas aeruginosa </it>PAO1. We examined this motility under several culture conditions, including inhibition of flagellar motility using Congo Red. We demonstrated that the presence of a wetting agent, mineral, and nutrient content of the media altered the swarming phenotype. We also demonstrated that the wetting agent reduces the surface tension of the agar. We were able to directly observe the presence of the wetting agent in the presence and absence of Congo Red, and found that incubation in a humidified chamber inhibited the production of wetting agent, and also slowed the progression of the swarming colony. We observed that swarming was related to both carbon and nitrogen sources, as well as mineral salts base. The phosphate concentration of the mineral base was critical for growth and swarming on glucose, but not succinate. Swarming on other carbon sources was generally only observed using M9 salts mineral base. Rapid swarming was observed on malic acid, d-sorbitol, casamino acids, and succinate. Swarming at a lower but still detectable rate was observed on glucose and sucrose, with weak swarming on maltose. Nitrogen source tests using succinate as carbon source demonstrated two distinct forms of swarming, with very different macroscopic swarm characteristics. Rapid swarming was observed when ammonium ion was provided as nitrogen source, as well as when histidine, tryptophan, or glycine was provided. Slower swarming was observed with methionine, arginine, or tyrosine. Large effects of mineral content on swarming were seen with tyrosine and methionine as nitrogen sources. Biofilms form readily under various culture circumstances, and show wide variance in structure under different conditions. The amount of biofilm as measured by crystal violet retention was dependent on carbon source, but not nitrogen source. Filamentous growth in the biofilm depends on shear stress, and is enhanced by continuous input of nutrients in chemostat culture.</p> <p>Conclusion</p> <p>Our studies have established that the beta-proteobacterium <it>Variovorax paradoxus </it>displays a number of distinct physiologies when grown on surfaces, indicative of a complex response to several growth parameters. We have identified a number of factors that drive sessile and motile surface phenotypes. This work forms a basis for future studies using this genetically tractable soil bacterium to study the regulation of microbial development on surfaces.</p

Self-Secured PUF: Protecting the Loop PUF by Masking

Physical Unclonable Functions (PUFs) provide means to generate chip individual keys, especially for low-cost applications such as the Internet of Things (IoT). They are intrinsically robust against reverse engineering, and more cost-effective than non-volatile memory (NVM). For several PUF primitives, countermeasures have been proposed to mitigate side-channel weaknesses. However, most mitigation techniques require substantial design effort and/or complexity overhead, which cannot be tolerated in low-cost IoT scenarios. In this paper, we first analyze side-channel vulnerabilities of the Loop PUF, an area efficient PUF implementation with a configurable delay path based on a single ring oscillator (RO). We provide side-channel analysis (SCA) results from power and electromagnetic measurements. We confirm that oscillation frequencies are easily observable and distinguishable, breaking the security of unprotected Loop PUF implementations. Second, we present a low-cost countermeasure based on temporal masking to thwart SCA that requires only one bit of randomness per PUF response bit. The randomness is extracted from the PUF itself creating a self-secured PUF. The concept is highly effective regarding security, low complexity, and low design constraints making it ideal for applications like IoT. Finally, we discuss trade-offs of side-channel resistance, reliability, and latency as well as the transfer of the countermeasure to other RO-based PUFs